TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| X. albilineans FL07-1 | Wild-type strain isolated in Florida | 47 |
| L. crescens BT-1 | Wild-type strain originally isolated from Babaco mountain papaya | 11 |
| L. crescens Sut-1 | sut::pCR2.1-TOPO derived from BT-1; Kanr | This study |
| L. crescens LcrI-1 | lcrI::pCR2.1-TOPO derived from BT-1; Kanr | This study |
| Plasmids | ||
| pCR2.1-TOPO | 3.9 kb; PCR cloning vector; Ampr Kanr | Invitrogen |
| pUFR071 | 9.4 kb; repW, ColE1, Mob+, LacZ+, Par+; Cmr Gmr | 27 |
| pMJ001 | Partial fragment of Lcr restriction subunit R lcrI (B488_RS03405) in pCR2.1-TOPO | This study |
| pMJ051 | Lcr gloA (B488_RS02175, with KpnI/HindIII ends) in pCR2.1-TOPO | This study |
| pMJ052 | KpnI/HindIII fragment from pMJ051 subcloned in pUFR071 | This study |
| pMJ053 | Xal gloA (XALXAFL071_RS04055, with EcoRI/HindIII ends) in pCR2.1-TOPO | This study |
| pMJ054 | EcoRI/HindIII fragment from pMJ053 subcloned in pUFR071 | This study |
| pMJ055 | CLas nttA (CLIBASIA_RS01005, with EcoRI/HindIII ends) in pCR2.1-TOPO | This study |
| pMJ056 | EcoRI/HindIII fragment from pMJ055 subcloned in pUFR071 | This study |
| pMJ057 | Partial fragment of Lcr gloA (B488_RS02175) in pCR2.1-TOPO | This study |
| pMJ058 | Partial fragment of Lcr sugar transporter sut (B488_RS00965) in pCR2.1-TOPO | This study |
a
Amp, ampicillin; Kan, kanamycin; Cm, chloramphenicol; Gm, gentamicin; Str, streptomycin. CLas, Lcr, and Xal represent “Ca. Liberibacter asiaticus,” L. crescens, and X. albilineans, respectively.