FIG 3.

Comparison of BGC transcription and metabolite accumulation in HD and C cultivation. (A) Relative transcription of BGCs in HD versus C cultivation. All transcript levels were normalized to the rnpB housekeeping gene. Numbers relate to the BGC identifiers of the AntiSMASH platform as listed in Table 1 The relative transcript level range between −0.5 and 2 is shaded in gray. (B) HPLC profiles of cellular extracts and supernatants of N. punctiforme PCC 73102 from HD and C cultures. The results of MALDI-TOF analysis of the peaks 1 to 6 are shown in Fig. S1 in the supplemental material. Peaks: 1, nostopeptolide 1052; 2, nostopeptolide A; 3, nostamide A; 4 to 6, unknown. AU, absorbance units.