TABLE 2.

Biosynthetic gene clusters and primers used for the detection of their cDNA in rtPCR

Cluster no. (AntiSMASH)	Namea	Primer	Target gene		Primer sequence (5′–3′)		PCR product size (bp)
			CyanoBaseb	AntiSMASH	Left	Right
3	pks1	C3	R2082	10475	CGTGGTTGACTGGAGATGCT	AAGCCTCTCGTGCCGTTTTA	108
4	Nos	nosA_RT4	F2181	10970	GTTTGCCCTCTCTGCTGAAC	GCGGTAAAGCAGGGTATCAA	109
5	Apt	apt_RT	F2460	12350	GAAATTGAGGCGCTTTTGAG	GGCTAGTGACGCTCACATCA	136
8	nrps1	C8	R3027	15385	AGCGGCAACATATTCCCCAA	ACGCCCAACCTGCTCTATTC	113
9	pks2	C9	F3163	16005	AAATCTTCGCCAGCATCCCA	TTGGCAACCTCCCCTTGTAC	101
10	ripp1	C10r	F3226	16320	GCAACTCTATAGCGCTCAAGG	GCTGTCTCTCCTGCATGACA	107
		C10L	R3212	16255	GGCAGAATTGGGAGGACGAA	TCCCAAACCCATCATTGAGCA	111
11	ripp2	C11a	R3313	16795	AGCAGACATCATAGCTCCACT	GGGTGCAGAAAAGGGCTACA	130
12	pks3	C12	F3359	17020	AGCTTGATGTTGTCTCCGCA	CTTCACCACGGACGTAACCA	115
13a	ripp3	C13a	R3395	17180	GGGTGGCCATACAACTCCAT	ATCTGCCGATGGTGAAGGTG	118
13b	pks4	C13b	R3445	17420	GGTGCAACCCGAAATCACAC	CAATGCTGGCTGTTCCCCTA	110
15	ripp4	C15	F5047	25520	TGATGGGGAAGCAACAGGTG	GAGGCGTTCAAGTTCGATGC	128
17	ripp5	C17	F6115	31000	GAGCGTGGTTATGACTTCACA	GTGCCGTCTAGTTCCCCATC	108
20	pks5	C20	R6590	33540	GGGGAATGGAAAGCATGGGA	ATTAACGCCCCTTCCCTGTG	125
pC1	nrps2	Cp	CR074	CR074	CTCATGTCGGGTGCAGCTTA	CCTCAATCCAAGTCAGGCGT	136
		rnpB_RT3	R018		GCGGTTGCAGATCAGTCATA	TCTGTGGCACTATCCTCACG	110

^aAs previously reported by Liaimer et al. (12).

^bDatabase accession numbers according to CyanoBase (http://genome.microbedb.jp/cyanobase).