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. 2001 Aug 28;98(18):10061–10066. doi: 10.1073/pnas.181348298

Figure 2.

Figure 2

Coimmunoprecipitation of SERCA1a and SERCA1a mutants with PLNΔ21–29 and PLNΔ21–30. (A) Microsomal fractions from HEK-293 cells coexpressing SERCA1a with either PLNΔ21–29 or PLNΔ21–30 were dissolved in Tween 20, and specific proteins were coimmunoprecipitated with anti-PLN antibody IDII. The immunoprecipitates were separated by SDS/PAGE, transferred to nitrocellulose membranes, and stained with anti-SERCA1a antibody A52. The interaction between SERCA1a and the PLN mutant PLNΔ21–29, but not PLNΔ21–30, is enhanced 4.5-fold. The relative levels of expression of SERCA1a (B) or of PLN and PLN deletion mutants (C) are shown. m, monomer; p, pentamer. (D). Microsomal fractions from HEK-293 cells coexpressing SERCA1a mutants N810A, D813A, or R822A with either PLNΔ21–29 or PLNΔ21–30 were analyzed in the same way. The interaction between SERCA1a mutant D813A and the PLN mutant PLNΔ21–29, but not PLNΔ21–30, is enhanced 1.7-fold.