Table 2.
Relative physical interactions between PLN domain IB mutants and SERCA1a L67 mutants D813A and R822A
| wt
|
D813A
|
R822A
|
||||||
|---|---|---|---|---|---|---|---|---|
| Mean ± SE | n | Mean ± SE | wt/D813A | n | Mean ± SE | wt/R822A | n | |
| wtPLN | 1 | 3 | 0.32 ± 0.07* | 3.1 | 4 | 0.64 ± 0.07* | 1.6 | 4 |
| R25A | 1.10 ± 0.05 | 3 | 0.54 ± 0.13* | 2.0 | 4 | 0.76 ± 0.12* | 1.4 | 4 |
| Q26A | 1.61 ± 0.18* | 3 | 0.86 ± 0.09 | 1.9 | 4 | 1.05 ± 0.12 | 1.5 | 4 |
| N27A | 2.64 ± 0.18* | 3 | 0.36 ± 0.06* | 7.3 | 4 | 1.03 ± 0.15 | 2.6 | 4 |
| L28A | 2.31 ± 0.25* | 3 | 0.84 ± 0.16 | 2.7 | 4 | 0.82 ± 0.20 | 2.8 | 4 |
| Q29A | 2.42 ± 0.28* | 3 | 1.00 ± 0.07 | 2.4 | 4 | 0.95 ± 0.08 | 2.6 | 4 |
| N30A | 2.95 ± 0.19* | 3 | 0.51 ± 0.06* | 5.8 | 4 | 1.15 ± 0.35 | 2.6 | 4 |
| I40A | 4.34 ± 0.64* | 3 | 1.60 ± 0.15* | 2.7 | 4 | 2.02 ± 0.65* | 2.2 | 4 |
The density of bands of wt SERCA1a or L67 mutants D813A and R822A coimmunoprecipitated with PLN domain IB mutants was measured, and the mean ± SE was calculated for each sample. Statistical analysis was carried out using Student's unpaired t test.
*
, P < 0.05 vs. coexpression of wt PLN and SERCA1a.