SDS/PAGE of (His)6-Y2:Y4 heterodimer in apo and holo forms reveal the stability of heterodimer through two chromatographic steps. Lane 1, low MW SigmaMarker; lane 2, apo (His)6-Y2:Y4 after TALON column; lane 3, apo complex after DEAE Sepharose column; lanes 4 and 5, holo (His)6-Y2:Y4 after TALON and DEAE Sepharose columns, respectively.