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. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: ACS Appl Mater Interfaces. 2017 Oct 20;9(43):37587–37596. doi: 10.1021/acsami.7b10592

Figure 3.

Figure 3

Conjugation of ACPP to microbubbles (MBs). (A) Schematic representation of the chemical constituents of Fl-ACPP-MBs. The Fl-labeled ACPP (green star) attaches to MBs through a thiol–maleimide coupling reaction. (B) Flow cytometry data of MBs before (left panel) and after (right panel) conjugation with Fl-ACPP showing the characteristic scatter plot of MBs (left) and the increase in fluorescence intensity (right) after conjugation. (C) Bright field (left) and fluorescence (right) microscopy of Fl-ACPP-MBs at 20×; scale bar is 50 μm.