Figure 5.
Fl-ACPP-MB cleavage at 37 °C when incubated with increasing thrombin concentrations (28, 56, 112, 196, and 280 nM) and with 280 nM thrombin plus hirudin. Percent of Fl-ACPP-MBs with fluorescence intensity above the initial MFI (Pre), immediately after adding thrombin (t = 0), and after 2, 5, 10, and 15 min of incubation (n = 3). Insert: dose–response effect of thrombin concentration on ACPP cleavage after 10 min of incubation.