Table 3.
Selected clones from each genetically identical or similar cluster were selected for testing for tolerance to [C2C1Im][OAc] and [C4C1Im]Cl ionic liquids
| TALEs | IL type | Concentration (%) | Gene set | Aver. growth rate (h−1) | Aver. final OD600 | Aver. lag-time (h) |
|---|---|---|---|---|---|---|
| MG 4.7 | [C4C1Im]Cl | 5.4 | MG-BM-3A | 0.26 ± 0.024 | 0.87 ± 0.039 | 4.88 ± 0.04 |
| MG 3.10 | [C4C1Im]Cl | 5.4 | MG-BM-3D | 0.31 ± 0.009 | 0.94 ± 0.04 | 10.85 ± 1.24 |
| MG 4.5 | [C4C1Im]Cl | 5.4 | MG-BM-3E | 0.30 ± 0.015 | 0.84 ± 0.082 | 3.19 ± 1.17 |
| MG 11.10 | [C2C1Im][OAc] | 4.6 | MG-EM-1 | 0.23 ± 0.003 | 1.69 ± 0.004 | 1.55 ± 0.006 |
| MG 12.7 | [C2C1Im][OAc] | 4.6 | MG-EM-1 | 0.27 ± 0.005 | 1.1 ± 0.03 | 1.9 ± 0.015 |
| MG 10.9 | [C2C1Im][OAc] | 4.6 | MG-EM-1A | 0.09 ± 0.003 | 0.74 ± 0.004 | 1.99 ± 0.006 |
| DH1 5.3 | [C4C1Im]Cl | 4.6 | DH-BM-3A | 0.32 ± 0.005 | 0.96 ± 0.01 | 12.51 ± 0.075 |
| DH1 5.10 | [C4C1Im]Cl | 4.6 | DH-BM-2 | 0.58 ± 0.023 | 0.61 ± 0.12 | 8.8 ± 1.059 |
| DH1 6.7 | [C4C1Im]Cl | 4.6 | DH-BM-1 | 0.36 ± 0.033 | 0.99 ± 0.094 | 5.62 ± 0.154 |
| DH1 7.5 | [C4C1Im]Cl | 4.6 | DH-BM-1 | 0.25 ± 0.025 | 1.39 ± 0.014 | 11.49 ± 3.604 |
| DH1 13.10 | [C2C1Im][OAc] | 4.2 | DH-EM-2A | 0.14 ± 0.013 | 0.52 ± 0.385 | 9.59 ± 0.66 |
| DH1 14.2 | [C2C1Im][OAc] | 4.2 | DH-EM-2B | 0.2 ± 0.027 | 0.36 ± 0.304 | 4.91 ± 1.083 |
| DH1 13.8 | [C2C1Im][OAc] | 4.2 | DH-EM-1 | 0.23 ± 0.007 | 0.6 ± 0.085 | 8.57 ± 3.393 |
| DH1 15.2 | [C2C1Im][OAc] | 4.2 | DH-EM-3C | 0.28 ± 0.057 | 0.57 ± 0.148 | 6.33 ± 0.259 |
| DH1 16.7 | [C2C1Im][OAc] | 4.2 | DH-EM-3A | 0.31 ± 0.014 | 0.26 ± 0.172 | 5.34 ± 0.44 |
Each of the TALE-derived isolates is presented with the corresponding IL-type and concentration in which it was originally evolved along with phenotypic characteristics of each of the selected isolates