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Reovirus replication in MEFs is facilitated by the expression of Atg3. Atg3wt, Atg3−/−, and Atg3+/+ MEFs were infected with R124 or jin-3 at an MOI of 0.1 or 10. After 48 h, protein lysates were prepared in Giordano lysis buffer and analyzed for the expression of σ3, Atg3, and Actin. In parallel, cells were collected in the supernatant, lyzed by three freeze-thaw cycles, and the total amount of infectious virus particles in the 24-wells plate well was determined by plaque assays.
