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Proliferation of T lymphocytes from immunized mice. Lymphocyte proliferation was determined by 3-(4,5-dimethylthylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay with recombinant core neutralizing epitope (COE) protein (a) or D protein (b) as stimulating agents and concanavalin A (ConA) as a positive control. * p < 0.05, ** p < 0.01 vs. phosphate-buffered saline (PBS) and vector control groups.
