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. 2017 Sep 30;9(10):285. doi: 10.3390/v9100285

Figure 5.

Figure 5

PIAS2 influences the degradation of HCV proteins through the proteasome pathway. (A–C) 293T cells were transfected with the indicated HCV protein expression plasmid, pCer-SUMO1 and pHA-Vector or pHA-PIAS2 and incubated for 48 h. Then, the cells were treated with MG-132 or chloroquine (CQ) for 4 h, and the protein expression levels were detected by western blotting; (D) Huh7 cells were transfected with either pHA-Vector or pHA-PIAS2 and infected with J399EM at an MOI of 0.1 for 72 h. After 4 h MG132 or chloroquine treatment, the expression levels of the HCV proteins were evaluated. The numbers below each blot show the relative density of the blots normalized to actin. DMSO: dimethyl sulfoxide.