Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The influence of PIAS2 on HCV protein ubiquitination. (A) 293T cells were transfected with the indicated plasmids together with Ub and Cer-SUMO1 expression plasmids. MG132 treatment was performed 4 h before the total protein was extracted. Then, immunoprecipitation and western blotting were performed; (B–D) 293T cells were transfected with the siRNA and plasmids as indicated. Total protein was collected and detected by western blotting, WCL: whole cell lysate.
