Figure 2.

Distribution of nsPs expressed in transfected cells. BSR cells were transfected with the replicase plasmid(s) indicated at the top and Tmed template plasmid. At 16 h post transfection, PNS was prepared and fractionated into P15 and S15, and nsPs were detected by Western blotting with specific antibodies. The polyprotein P123 was recognized using anti-nsP3 antibody. β-actin was used as a cytosolic marker. Equivalent amounts of PNS, P15, and S15 were loaded. Two independent experiments were performed and one representative result is shown here.