Figure 3.

Distribution and stability of endogenous minus- and plus-strand RNA in P15 and S15 fractions prepared from transfected BSR cells. (A) PNS was prepared at 16 h post transfection, and total RNA was isolated from equivalent amounts of PNS, P15, and S15 followed by in-gel hybridization with probes that detect either minus- or plus-strand RNA. Subgenomic (SG) RNA is indicated by Tmed SG. Numbers below the lanes indicate the percentage of ³²P-label detected in the genomic RNA compared to PNS. For the plus-strand RNA, the genomic Tmed was quantified. As a control, a probe against ribosomal 18S RNA was used; (B) PNS, P15, and S15 from cells co-transfected with the P1^2^34 and Tmed constructs were treated with RNase A/T1 under low salt conditions, and RNA was detected as in A. Numbers below the lanes indicate the percentage of ³²P-signal in genomic RNA compared to the untreated sample. Representative experiments are shown.