Figure 6.

P4^PAV and P4^PAS reduce the accumulation of siRNAs. GFP-expressing N. benthamiana 16c leaves were co-infiltrated with sense GFP plus the empty vector, TBSV P19, PLRV P0 (P0^PL) or RYMV P1 (P1^RY) as controls, or BYDV-PAV P4 (P4^PAV), BYDV-PAS P4 (P4^PAS) or PLRV P4 (P4^PL). P4^PAV was also infiltrated at a five-fold dilution (*P4^PAV). Non-infiltrated plants (16c) were also included as negative controls. Pictures were taken under the UV light and leaf samples collected for Northern blots at 6 dpi. Total RNA (20 μg) was blotted and probed for the presence of GFP mRNA. rRNA was used as a loading control for high-molecular-weight RNA. Total RNA (40 μg) from the agro-infiltrated tissues was also blotted and probed for the presence of GFP siRNAs. U6 was used as a loading control for low-molecular weight RNA. RNA size markers are indicated.