Figure 1.

Read distribution in samples analyzed for virion-incorporated transcripts. Highly pure cowpox virus (CPXV) intracellular mature virion (IMV) particles passaged in (a) HEp-2 or (b) Rat-2 cells were analyzed for transcripts by next-generation sequencing (NGS). Reads mapping to the virus genome and the host genome (human or rat) were sorted by read count (RPKM = reads per kilobase million). Dashed lines: cutoff 90%. Three different protocols were applied for sample preparation: I: RNase digestion, II: no RNase digestion and III: protease and RNase digestion.