Figure 3.

Western blot analysis of NS1 in H-1PV-infected osteosarcoma cells. Osteosarcoma cells were either mock-treated or infected with wild-type H-1PV (MOI: 1 plaque-forming units (PFU) per cell). Cell lysates from cultures collected at the indicated times were processed for western blotting with NS1-specific primary antiserum and horseradish-peroxidase-conjugated secondary antibodies. Blots were revealed by chemiluminescence. In H-1PV-infected cell lysates, two NS1-specific bands of approximately 60 kDa were detected. GAPDH was used as loading control. Results for SaOS-2 and U2-OS are shown as examples.