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. 2017 Oct 17;9(10):301. doi: 10.3390/v9100301

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The efficiency of wtH-1PV replication in osteosarcoma cells is limited. Cells were infected with one infectious particle per cell (PFU/cell) and cell supernatants were collected at the indicated times. (A) The number of viral genome copies released was determined into the cell culture medium in triplicate by quantitative polymerase chain reaction (qPCR) (copies/mL), and means are shown. Error bars represent SEM (upper panel); (B) titers of infectious particles (RU/mL) were determined by infecting NBK324K cells with serial dilutions of the cell culture supernatant and by dot blot detection of viral DNA 72 h after infection. Error bars represent SEM (lower panel).