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. 2017 Sep 26;9(10):1066. doi: 10.3390/nu9101066

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of ferulic acid on protein synthesis of fast skeletal myofibers in adult zebrafish. (a) Western blot and (b) fold change of relative intensity of zTOR, Phospho-zTOR (Ser2448); (c) Western blot and (d) fold change of relative intensity of p70S6K, Phospho-p70S6K (Ser371); (e) Western blot and (f) fold change of relative intensity of 4E-BP1, Phospho-4E-BP1(Thr37/46); and, (g) Western blot and (h) fold change of relative intensity of myosin heavy chain, MyHC (fast). The relative intensity of all proteins was calculated after normalizing with GAPDH. Data from the FA group are shown as fold changes after being normalized to the NC group. NC: negative control group; and, FA: ferulic acid administered group. Data are expressed as mean ± SD (n = 5, per group from population 2), * p < 0.05, ** p < 0.01 compared to the NC group.