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. 2017 Sep 27;9(10):1070. doi: 10.3390/nu9101070

Figure 7.

Figure 7

Representative high-performance liquid chromatography (HPLC) chromatograms of aqueous Cynanchum wilfordii extract (CWW). Mobile phase consisted of solvents A (methanol) and B (0.1% formic acid) run at a flow rate of 1.0 mL/min. Elution conditions were as follows: 0–5 min, 20% A; 5–12 min, 20–30% A; 12–20 min, 30–40% A; 20–25 min, 40–80% A; 25–35 min, 80–100% A; 35–46 min, 100% A; 46–50 min, 100–20% A; 50–60 min, 20%; and finally, the column was washed and reconditioned. Sample injection volume was 20 μL. Optimum HPLC separation was achieved at 35 °C and monitored at 284 nm. AU: arbitrary unit.