Figure 1.

Cytomorphological view of MDA-MB-231 breast cancer cells without any treatment (a–d) as well as after 24 h of caffeic acid (CA) (e,f) and 24 h of caffeic acid phenethyl ester (CAPE) treatment (g,h), both with a 50 µM dose. To prepare the samples a hematoxylin and eosin staining was used. Exposition: optical magnification ×100 (a,e,g), ×400 (c,d,f,h), ×600 (b). Main features: (a) phenotypically as spindle-shaped cells (caudate, tadpole), hyperchromasia; (b) irregular nuclear shapes, small clusters of cells around the large ones; (c) large nuclei with irregular shape and several nucleoli in the nucleus; (d) pleomorphism of size, shape, and coloration of nuclei and whole cells; (e) karyopyknosis; (f) lower cell-cell contact; (g) karyopyknosis, cytoplasm density; (h) cytoplasm density and a shape change, cytoplasm fragmentation.