Fig. 5.
Analysis of AuxREs in promotors and auxin-induced expression of B. napus Aux/IAA genes. a Distribution of AuxREs in the 2.0 kb regions upstream of translation initiation site. The location of translation initiation site was designed as +1 bp and the numbers of AuxREs were calculated at intervals of 500 bp. b Scatterplot showing the significantly differentially expressed Aux/IAA genes. The fold change of Aux/IAA genes expression in auxin treated seedlings relative to control was expressed as log2(foldchange) on the x-axis. The adjusted p value calculated by DEGseq program was expressed as -log10(q-value) on the y-axis. c Enrichment analysis of auxin up-regulation of Aux/IAA genes. The associations between presence of AuxREs and auxin up-regulation were analyzed by Pearson’s chi-square test where a p-value <0.05 indicates a significant association. The horizontal dotted gray line indicates a threshold of –log10(p value) = 1.30