Figure 1.

Oligonucleotide sequences. (A–E) Fluorescein-labeled double-stranded ODNs: 36-mer (A), 21-mer (B and C), 13-mer (D), and 7-mer (E). (F) Fluorescein-labeled single-stranded 19-mer. The preprocessed, double-stranded DNA was prepared by annealing equimolar amounts of complementary strands F19U5B and 21U5A. F designates the fluorescein probe. (G) 21-mer single-stranded ODN with 3MI (M) substituted for G. The 3MI-containing duplex was obtained by annealing PTER1 and 21U5A. Terminal bases removed by IN during the 3′ processing reaction are underlined.