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. 2001 Aug 14;98(18):10090–10095. doi: 10.1073/pnas.181024498

Figure 5.

Figure 5

Correlation time distributions of the IN-DNA complex determined by monitoring fluorescein-labeled ODNs. (A) F21U5B/21U5A alone at 25°C. (B) F21U5B/21U5A in the presence of 100 nM IN at 25°C. (C) F36U5B/36U5A in the presence of 100 nM IN at 25°C. (D) F36U5B/36U5A in the presence of 100 nM IN at 37°C. Excitation and emission wavelengths were 492 and 530 nm, respectively. The lifetime distribution, as recovered by quantified maximum entropy method analysis, was not influenced by the protein binding: τ1 = 0.3 ± 0.1 ns (8%); τ2 = 1.8 ± 0.1 ns (16%); τ3 = 3.9 ± 0.1 ns (76%).