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. 2001 Aug 21;98(18):10096–10101. doi: 10.1073/pnas.171319598

Figure 2.

Figure 2

pH-dependent changes in DMS reactivity at A2451 in 70S ribosomes under different conditions. Primer extension analysis of 23S rRNAs isolated from unmodified (DMS −) and DMS-modified (DMS +) ribosomes. A, C, and U are dideoxy sequencing lanes. (A) Influence of DMS incubation conditions: 70S ribosomes were modified with DMS either for 10 min at 37°C or for 1 h at 0°C. The asterisk denotes A2469, the modification of which is strongly enhanced at higher temperatures. (B) Influence of the activation of 70S ribosomes by preincubation for 15 min at 37°C (lanes 1–4) or 15 min at 0°C (lanes 5–8), before DMS modification (1 h at 0°C).