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. 2001 Aug 21;98(18):10108–10112. doi: 10.1073/pnas.181199698

Figure 3.

Figure 3

Dependence of NADH consumption on NO concentration. (A) HMP (0.2 μM) was incubated in buffer to give the indicated O2 concentrations and mixed with anaerobic buffer containing NADH (100 μM) and NO. (B) Linear NADH consumption at low pO2. The absorbance traces at 12.5 μM O2 from A for no NO (line 1), 1 μM NO (line 2), 2 μM NO (line 3), 5 μM NO (line 4), and 10 μM NO (line 5). Two molecules of NO are consumed per molecule of NADH. Conditions: phosphate buffer (pH 7, 100 mM), 0.1 mM EDTA, 0.2 cm pathlength, 10°C.