Fig. 1.

The phenotype of the Arabidopsis ntrc mutant is suppressed by decreased levels of 2-Cys Prxs. (A) WT and mutant lines were grown under short-day conditions for 9 wk. The weights of rosette leaves from at least seven plants are presented as average values ± SE. Letters indicate significant differences by Student’s t test at a 95% CI (B). (C) Western blot analysis of the content of NTRC and 2-Cys Prxs. Protein extracts were obtained from leaves of plants grown as stated in A, and aliquots of 15 μg (1×) from all lines, as well as 1:2, 1:4, and 1:8 dilutions from WT, as indicated, were subjected to SDS/PAGE under reducing conditions, transferred to nitrocellulose filters, and probed with anti-NTRC or anti-2-Cys Prxs antibodies. Even loading was monitored by Ponceau staining. Band intensities of 2-Cys Prxs and Ponceau were quantified (ScionImage), and the ratio between them, referred to the WT 1× sample (arbitrarily assigned a value of 100), are indicated.