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. 2017 Oct 24;114(45):12069–12074. doi: 10.1073/pnas.1706003114

Fig. S3.

Fig. S3.

The effect of the overexpression of 2-Cys Prx A and 2-Cys Prx B in Arabidopsis WT, ntrc mutant, and suppressed lines. Two independent transgenic lines, nos. 1 and 2, expressing 2-Cys Prx A or 2-Cys Prx B under the CaMV 35S constitutive promoter in the ntrc (A), WT (C), ntrc-Δ2cp (E), and ntrc-trxf1f2-Δ2cp (T1 generation; G) genetic backgrounds were grown under short-day (A, C, and E) or long-day (G) photoperiod for 8 wk (A and C) or 4 wk (E and G). (B, D, F, and H) Western blot analyses of the contents of NTRC, 2-Cys Prxs, and Trxs f (H) in WT, mutants, and transgenic lines. Protein extracts [2 µg (B), 4 µg (D), 3 µg (F and H)] from leaves of plants grown as stated earlier were subjected to SDS/PAGE under reducing conditions, transferred to nitrocellulose filters, and probed with anti-NTRC, anti-2-Cys Prxs, and anti-Trxs f antibodies. Even loading was monitored by Ponceau staining. Band intensities of 2-Cys Prxs and Ponceau were quantified (ScionImage), and the ratios between them, referred to the ntrc (B and F), WT (D), or ntrc-trxf1f2 (H) samples, arbitrarily assigned a value of 100, are indicated.