Fig. S5.

Bands detected by antibodies against Trxs f in MM(PEG)₂₄-based alkylation experiments and aggregation state of 2-Cys Prxs in WT and mutant lines. (A) WT and the trxf1f2 mutant of A. thaliana were grown under long-day conditions for 4 wk. Alkylation assays were performed as described in Materials and Methods using leaves harvested at the end of the night (marked as “D”) and after 30 min of illumination (“L”) with growth light intensity. Proteins were resolved in SDS/PAGE under nonreducing conditions, transferred to nitrocellulose filters, and probed with the anti-Trx f antibodies. ns, nonspecific; oxi, oxidized; red, reduced. (B) Western blot analysis of the aggregation state of 2-Cys Prxs. Protein extracts (15 μg) from leaves were subjected to native gel electrophoresis, transferred to nitrocellulose filters, and probed with anti–2-Cys Prxs antibodies. Even loading was monitored by Ponceau staining, and molecular weight markers (in kilodaltons) are indicated. a, aggregated; d, dimer.