Fig. S8.

Hydrogen peroxide content and expression of genes encoding chloroplast peroxidases in Arabidopsis WT and mutant plants. WT and mutant lines, as indicated, were grown under long-day and growth-light conditions for 4 wk. (A) The hydrogen peroxide content in whole rosettes (n ≥ 8) was measured as indicated in Materials and Methods. Mean values ± SE from two independent experiments combined are shown, with letters indicating significant differences by Tukey test at a 99% CI. (B and C) The levels of transcripts of the indicated genes, determined by quantitative RT-PCR using oligonucleotides indicated in Table S3, were normalized against three reference genes (Materials and Methods) and referenced against the levels of each of the genes in WT plants, arbitrarily considered as 100%. Determinations were performed three times, and mean values ± SE are presented.