Table S1.
Nonenzymatic antioxidant | WT | ∆2cp | ntrc | ntrc-∆2cp |
AsA+DHA (μmol⋅g−1 FW) | 3.806 ± 0.207 | 3.675 ± 0.260 | 3.334 ± 0.225 | 3.571 ± 0.276 |
AsA/(AsA+DHA) | 0.914 ± 0.019* | 0.922 ± 0.026* | 0.829 ± 0.017† | 0.883 ± 0.016*† |
GSH+GSSG (μmol⋅g−1 FW) | 0.924 ± 0.041 | 0.944 ± 0.064 | 0.890 ± 0.056 | 0.918 ± 0.032 |
GSH/GSH+GSSG | 0.879 ± 0.027* | 0.856 ± 0.023* | 0.796 ± 0.025† | 0.851 ± 0.035*† |
Whole rosettes (n = 5) of 6-wk-old plants grown under short-day photoperiod were harvested to determine AsA, DHA, GSH, and GSSG (Materials and Methods). For the indicated lines, total ascorbic acid (AsA+DHA), reduced ascorbic acid /total ascorbic acid (AsA/AsA+DHA) ratios, total glutathione (GSH+GSSG), and reduced glutathione/total glutathione (GSH/GSH+GSSG) ratios are presented as mean values ± SE from two independent experiments combined.
,†Significant differences between genotypes marked with different symbols (Tukey test at a 95% CI).