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. 2017 Oct 24;114(45):12069–12074. doi: 10.1073/pnas.1706003114

Table S1.

Determination of nonenzymatic antioxidants in Arabidopsis WT and mutant plants

Nonenzymatic antioxidant WT 2cp ntrc ntrc-∆2cp
AsA+DHA (μmol⋅g−1 FW) 3.806 ± 0.207 3.675 ± 0.260 3.334 ± 0.225 3.571 ± 0.276
AsA/(AsA+DHA) 0.914 ± 0.019* 0.922 ± 0.026* 0.829 ± 0.017 0.883 ± 0.016*
GSH+GSSG (μmol⋅g−1 FW) 0.924 ± 0.041 0.944 ± 0.064 0.890 ± 0.056 0.918 ± 0.032
GSH/GSH+GSSG 0.879 ± 0.027* 0.856 ± 0.023* 0.796 ± 0.025 0.851 ± 0.035*

Whole rosettes (n = 5) of 6-wk-old plants grown under short-day photoperiod were harvested to determine AsA, DHA, GSH, and GSSG (Materials and Methods). For the indicated lines, total ascorbic acid (AsA+DHA), reduced ascorbic acid /total ascorbic acid (AsA/AsA+DHA) ratios, total glutathione (GSH+GSSG), and reduced glutathione/total glutathione (GSH/GSH+GSSG) ratios are presented as mean values ± SE from two independent experiments combined.

*

,†Significant differences between genotypes marked with different symbols (Tukey test at a 95% CI).