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. 2017 Oct 24;114(45):12069–12074. doi: 10.1073/pnas.1706003114

Table S3.

Arabidopsis oligonucleotides used in this study

Gene Locus Sequence
Genotyping oligonucleotides
SALK T-DNAleft border T-DNA 5′-ATTTTGCCGATTTCGGAAC-3′
GABI T-DNAleft border T-DNA 5′-ATATTGACCATCATACTCATTGC-3′
ntrc (SALK_012208) At2g41680 5′-TCACCAACATGTGGCCC-3′
5′-TTCTTCATCTTCACACCCGA-3′
2cpa (SALK_065264) At3g11630 5′‐GAGAAGTTGAACACCGA‐3′
5′‐GGGGACAAAGTGAGAATC‐3′
2cpaGK (GABI_295C05) At3g11630 5′‐CTTCCACTGGTTGGAAACAAG‐3′
5′‐AATGCCTGCAACATTGAAAAC‐3′
2cpb (SALK_017213) At5g06290 5′‐CCACCTGAACCAAGAAAG‐3′
5′‐CCTGCAAGACAACATCAC‐3′
trx f1 (SALK_128365) At4g18480 5′-TTCATATGTGTAGCTTAGAAACCGTTAATG-3′
5′-AAGTCGACAGAGACTGGTTCATCCG-3′
trx f2 (GK-020E05-013161) At5g16400 5′-CTATTTCAACAATCGGGTCCC-3′
5′-TTCTCAATTGCCAATTCTTGC-3′
RT-qPCR oligonucleotides
ACTIN At3g18780 5′-GCACTTGCACCAAGCAGCAT-3′
5′-CCTTTCAGGTGGTGCAACGAC-3′
AT5G25760 At5g25760 5′-CTGCGACTCAGGGAATCTTCTAA-3′
5′-TTGTGCCATTGAATTGAACCC-3′
AT4G05320 At4G05320 5′-CTATTTCAACAATCGGGTCCC-3′
5′-AAAGAGATAACAGGAACGGAAACATAGT-3′
PRX IIE At3g52960 5′-GTTAGGTCAAGGAGATATGCGA-3′
5′-GAACTTTCACAACACCATCATCAG-3′
PRX Q At3g26060 5′-ATTGTTAAGCGACGAAGGGA-3′
5′-CAATGCTCCAAACAGGTCTC-3′
2-CYS PRX A At3g11630 5′-AGGTACAATCTTTGTTATATGTGAGC-3′
5′-GACAAAGTGAGAATCAAACAAAGG-3′
2-CYS PRX B At5g06290 5′-GCCAAAGAACCTTTGTATCTATCTC-3′
5′-TTCAATTTAAGGAGAATCACATAGCA-3′
sAPX At4g08390 5′-TCCTCCTTCACCTGCTACTC-3′
5′-TCCTTGTCATCTAATCCCATTCTG-3′
tAPX At1g77490 5′-GCCCAGATAAGCCATTACCC-3′
5′-GGAGTTGTTATTACCACCAAAGAG-3′
GPX1 At2g25080 5′-GTCAACAGTTCTAGACCTAATTCC-3′
5′-CAAGGAAGGGACGAGAAAGG-3′
GPX7 At4g31870 5′-TGCTTGCGGCTTAAATTTAGTG-3′
5′-CTGGTATACCAACAATAACCTGGA-3′
cDNA cloning oligonucleotides
Gene Plasmid Sequence
2-Cys Prx A pDONR-207 5′-AAAAAGCAGGCTtcATGGCGTCTGTTGCTTCT-3′
5′-AGAAAGCTGGGTcCTAAATAGCTGAGAAGTACTC-3′
2-Cys Prx B pDONR-207 5′-AAAAAGCAGGCTtcATGTCAATGGCGTCTATA-3′
5′-AGAAAGCTGGGTcCTAGATAGCTGAAAAGTATTC-3′

Additional nucleotides included to maintain the proper reading frame are underlined.