Table S5.
Comparison of assembly results for five cacti in this study
| Species | C. gigantea | P. pringlei | L. schottii | S. thurberi | P. humboldtii |
| Flow cytometry est. genome size (Mb, Kew Database) | 1,403 | — | — | 1,682 | — |
| In silico estimated genome size, Mb | 1,303 | 1,410 | 1,474 | 1,420 | 980 |
| Meraculous k-mer value | 69 | 37 | 55 | 57 | 41 |
| No. of scaffolds | 57,409 | 171,584 | 158,705 | 159,478 | 126,352 |
| Total size of scaffolds, bp | 980,358,000 | 629,656,250 | 797,927,173 | 853,349,426 | 414,047,441 |
| Longest scaffold, bp | 648,566 | 68,912 | 148,470 | 151,404 | 58,930 |
| Shortest scaffold, bp | 1,000 | 200 | 200 | 200 | 201 |
| No of contigs > 1 kb | 57,378 | 165,982 | 155,005 | 156,573 | 124,110 |
| Percentage of contigs > 1 kb | 100 | 96.7 | 97.7 | 98.2 | 98.2 |
| No. of contigs > 10 kb | 17,976 | 10,469 | 19,671 | 21,527 | 4,154 |
| Percentage of contigs > 10 kb | 31.3 | 6.1 | 12.4 | 13.5 | 3.3 |
| No. of contigs > 100 kb | 1,893 | 0 | 11 | 20 | 0 |
| Percentage of contigs > 100 kb | 3.3 | 0.0 | 0.0 | 0.0 | 0.0 |
| Mean scaffold size, bp | 17,077 | 3,670 | 5,028 | 5,351 | 3,277 |
| Median scaffold size, bp | 3,368 | 2,347 | 2,559 | 2,575 | 2,338 |
| N50 scaffold length, bp | 61,546 | 5,411 | 9,302 | 10,456 | 4,395 |
| L50 scaffold count | 4,575 | 32,562 | 21,671 | 20,352 | 28,266 |
| scaffold %A | 31.06 | 30.71 | 31.26 | 31.34 | 31.43 |
| scaffold %C | 18.08 | 17.32 | 17.99 | 18 | 17.69 |
| scaffold %G | 18.05 | 17.15 | 17.86 | 17.86 | 17.48 |
| scaffold %T | 30.87 | 29.73 | 30.49 | 30.59 | 30.38 |
| scaffold %N | 1.93 | 5.09 | 2.39 | 2.21 | 3.01 |
| Percentage of assembly in scaffolded contigs | 76.0 | 65.3 | 66.3 | 76.3 | 67.5 |
| Percentage of assembly in unscaffolded contigs | 24.0 | 34.7 | 33.7 | 23.7 | 32.5 |
| Average number of contigs per scaffold | 1.9 | 1.9 | 1.9 | 2.2 | 2.1 |
| Average length of break (>25 Ns) between contigs in scaffold | 374.1 | 194.6 | 128.9 | 83.4 | 81.7 |
| No. of contigs | 107,828 | 333,727 | 300,317 | 358,224 | 261,756 |
| No. of contigs in scaffolds | 67,765 | 247,789 | 210,664 | 277,545 | 198,246 |
| No. of contigs not in scaffolds | 40,063 | 85,938 | 89,653 | 80,679 | 63,510 |
| Total size of contigs, bp | 961,496,166 | 598,104,997 | 779,678,560 | 836,766,707 | 402,983,040 |
| Longest contig, bp | 351,939 | 46,681 | 81,565 | 62,746 | 19,699 |
| No. of contigs > 1 kb | 98,083 | 192,939 | 201,546 | 230,699 | 156,032 |
| Percentage of contigs > 1 kb | 91 | 57.8 | 67.1 | 64.4 | 59.6 |
| No. of contigs > 10 kb | 28,004 | 4,039 | 12,663 | 10,821 | 383 |
| Percentage of contigs > 10 kb | 26 | 1.2 | 4.2 | 3 | 0.1 |
| No. of contigs > 100 kb | 216 | 0 | 0 | 0 | 0 |
| Percentage of contigs > 100 kb | 0.2 | 0.0 | 0.0 | 0.0 | 0.0 |
| Mean contig size, bp | 8,917 | 1,792 | 2,596 | 2,336 | 1,540 |
| Median contig size, bp | 3,904 | 1,172 | 1,423 | 1,361 | 1,191 |
| N50 contig length, bp | 20,427 | 3,174 | 5,046 | 4,402 | 2,335 |
| L50 contig count | 12,608 | 52,530 | 40,107 | 52,163 | 53,282 |
| BUSCO gene models | |||||
| Complete Single-copy no., % | 870 (91) | 592 (61) | 714 (74) | 688 (71) | 503 (52) |
| Complete Duplicated no., % | 253 (26) | 172 (17) | 217 (22) | 197 (20) | 128 (13) |
| Fragmented no., % | 35 (3.6) | 198 (20) | 128 (13) | 148 (15) | 262 (27) |
| Missing no., % | 51 (5.3) | 166 (17) | 114 (11) | 120 (12) | 191 (19) |
| NCBI accession no. | NCQR00000000 | NCQS00000000 | NCQV00000000 | NCQT00000000 | NCQU00000000 |