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. 2017 Oct 23;114(45):11920–11925. doi: 10.1073/pnas.1711482114

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Fig. 4. — Analysis of fluorothreonine physiological markers in WT, ΔfthB::Am^R, and ΔfthC::Am^R S. cattleya strains after onset of organofluorine production (6 d). (A) Levels of secreted fluoroacetate and fluorothreonine in culture supernatant measured by ¹⁹F NMR. (B) Intracellular amino acid levels in cell pellet lysates measured by LC-MS/MS. (C) Fluorothreonyl-tRNA abundance compared with total fluorothreonyl- and threonyl-tRNA measured by RNase digestion followed by LC-MS/MS. (D) Incorporation of fluorothreonine into the total protein fraction measured by LC-MS/MS analysis of total protein hydrolysate. Data are mean ± SE (n = 4).