Table 2.

Assessment of fluorothreonine incorporation across the proteome of S. cattleya measured by shotgun proteomics of WT and ΔfthB S. cattleya strains

Strain	PSM or protein	FThr	Total
WT	PSMs	20 ± 10	25,000 ± 3,000
	Proteins	9 ± 2	1,630 ± 90
ΔfthB	PSMs	1,800 ± 300	24,000 ± 3,000
	Proteins	300 ± 20	1,600 ± 100

Database searches were performed with FThr for Thr substitution as a variable modification. The number of fluorothreonine-containing and total peptide spectrum matches and proteins IDs were then tabulated. All measurements were performed with n = 4; values are reported as mean ± SE.