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. 2017 Oct 23;114(45):E9722–E9729. doi: 10.1073/pnas.1714422114

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Fig. 3. — BiFC of PtrVND6-C1^IR with each of the PtrVND6 family members. PtrVND6-C1^IR was fused with CFP^C, and each of the PtrVND6 family members was fused with CFP^N. CFP signal was detected in the nucleus of the SDX protoplasts transfected by H2A:mCherry and PtrVND6-C1^IR:CFP^C with (A) PtrVND6-A1:CFP^N, (C) PtrVND6-A2:CFP^N, (E) PtrVND6-B1:CFP^N, (G) PtrVND6-B2:CFP^N, (I) PtrVND6-C1:CFP^N, (K) PtrVND6-C2:CFP^N, or (M) PtrVND6-C1^IR:CFP^N. SDX protoplasts transfected with only (B) PtrVND6-A1:CFP^N, (D) PtrVND6-A2:CFP^N, (F) PtrVND6-B1:CFP^N, (H) PtrVND6-B2:CFP^N, (J) PtrVND6-C1:CFP^N, (L) PtrVND6-C2:CFP^N, or (N) PtrVND6-C1^IR:CFP^C were used as negative controls. The diameter of the SDX protoplasts is ∼30 μm.