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. 2017 Oct 23;114(45):E9722–E9729. doi: 10.1073/pnas.1714422114

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Fig. 6. — BiFC of PtrVND6-C1^IR with each of the PtrSND1 family members and PtrSND1-A2^IR with each of the PtrVND6 family members in SDX protoplasts. PtrVND6-C1^IR and PtrSND1-A2^IR were fused with CFP^C, and full-length PtrVND6 and PtrSND1 members were fused to CFP^N. H2A was fused with mCherry as a nuclear marker. CFP signal was detected in the nucleus of the SDX protoplasts transfected by H2A:mCherry with the following combinations: (A) PtrSND1-A1:CFP^N, (C) PtrSND1-A2:CFP^N, (E) PtrSND1-B1:CFP^N, or (G) PtrSND1-B2:CFP^N each with PtrVND6-C1^IR:CFP^C; (I) PtrVND6-A1:CFP^N, (K) PtrVND6-A2:CFP^N, (M) PtrVND6-B1:CFP^N, (O) PtrVND6-B2:CFP^N, (Q) PtrVND6-C1:CFP^N, or (S) PtrVND6-C2:CFP^N each with PtrSND1-A2^IR:CFP^C. No CFP signal was detected from SDX transfected with only (B) PtrSND1-A1:CFP^N, (D) PtrSND1-A2:CFP^N, (F) PtrSND1-B1:CFP^N, (H) PtrSND1-B2:CFP^N, (J) PtrVND6-A1:CFP^N, (L) PtrVND6-A2:CFP^N, (N) PtrVND6-B1:CFP^N, (P) PtrVND6-B2:CFP^N, (R) PtrVND6-C1:CFP^N, or (T) PtrVND6-C2:CFP^N. The diameter of the SDX protoplasts is ∼30 µm.