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. 2017 Oct 23;114(45):E9722–E9729. doi: 10.1073/pnas.1714422114

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Fig. S5. — Western blot analysis using SDX nuclear protein shows that PtrVND6-C1^IR can be detected but PtrVND6-A1^IR cannot be detected. Antibody specificity test of (A) NAC domain of PtrVND6-A1 and PtrVND6-A1^IR, (B) NAC domain of PtrVND6-C1 and PtrVND6-C1^IR, and (C) 28 aa of PtrVND6-C1^IR using recombinant proteins of PtrVND6-A1, -A2, -B1, -B2, -C1, -C2, -A1^IR, -A2^IR, and -C1^IR. (D) Antibody specific to the NAC domain of PtrVND6-A1 and -A1^IR detected only PtrVND6-A1 in SDX nuclear protein. Recombinant protein of PtrVND6-A1^IR was used as the control. (E) Antibody specific to the NAC domain of PtrVND6-C1 and -C1^IR detected both PtrVND6-C1 and -C1^IR in SDX nuclear protein. (F) Antibody specific to the 28 aa of PtrVND6-C1^IR detected PtrVND6-C1^IR in SDX nuclear protein. Recombinant protein of PtrVND6-C1^IR was used as the control in E and F.