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TNR-SR2 mediates nuclear import of phosphorylated SR proteins. GST-ASF and -RS were in vitro phosphorylated or mock-treated by SRPK1. The nuclear import assay for these two GST-SR protein fusions was carried out in a bacterial extract containing TRN-SR2 (+TRN-SR2) or in mock extract (−TRN-SR2). Effects of low temperature (d and i) or of RanQ69L-GTP (0.1 mg/ml) (e and j) on import were examined.
