Skip to main content
. 2001 Aug 21;98(18):10154–10159. doi: 10.1073/pnas.181354098

Figure 5.

Figure 5

TRN-SR2 interacts with nucleoporin p62 and is targeted to nuclear speckles. (A) GST or GST-transport receptor fusions (2 μg each) were incubated with HeLa cytosol, as described in Materials and Methods. Proteins interacting with GST or GST-fusion transporters were selected by glutathione-Sepharose and detected by immunoblotting with mAb 414 (lanes 1–5) or an antibody to p62 (lanes 6–9). For competition, binding reaction mixtures contained transport buffer alone (−; lane 7), 10 μM phosphorylated SR peptide (SR; lane 8), or 5 μM RanQ69L-GTP (Ran; lane 9). Lane 1 shows 6.6% of the input into the pull-down assay. The asterisk represents a band that was detected in some but not all batches of the cytosol used. (B) Permeabilized HeLa cells were incubated with 0.6 μM GST-TRN-SR2 or GST-impβ in the presence of an ATP regeneration system. Double-label immunofluorescence was performed by using polyclonal anti-GST (a and d) and monoclonal anti-SC35 (b and e) antibodies. Merged images are shown in c and f.