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. 2017 Sep 26;6:e28716. doi: 10.7554/eLife.28716

Figure 4. Pom121 membrane topology and rotational mobility of the mEos3 probe within the FG-network under various conditions.

Figure 4.

(A) Possible membrane topologies of Pom121. The N-terminus of Pom121 is predicted to be in the lumen of the ER (1 TMD) or in the central pore (2 TMDs). TMD = transmembrane domain. (B) Var(p)cir values for various proteins under the indicated conditions. p-PALM data were obtained under the same conditions as in Figure 3. The stars (*) indicate significantly different values from the wildtype (blue) condition within the same group according to a two-sided Welch’s t-test (95%). Note that the actual significance level is expected to be higher than indicated since the error bars shown here are wider than a typical standard deviation (see Figure 2—figure supplement 6). A significant effect of WGA on the probe attached to the N-terminus of Pom121 suggests that this part of Pom121 is located within the central pore, and not in the ER lumen, and thus, that Pom121 has two TMDs (see (A)). 10 µM NTR ≈ 1 mg/mL.