Video 1. p-PALM imaging of Pom121-mEos3 at the bottom of the nucleus.

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DOI: 10.7554/eLife.28716.006

The top half is the p-channel and the bottom half is the s-channel. The round illumination area created by the narrow-field epifluorescence imaging is clearly detectable within the center of the fields. Fluorescent spots that appear and disappear arise from single mEos3 molecules and are clearly correlated between the two channels. t = 10 ms; 240 nm square pixels (see Figure 1).