Table 1.
Antibodies used in the protocol
| Primary antibody | Species | Blocking buffer | Dilution | Secondary antibody (1:500) |
|---|---|---|---|---|
| Nkx2.5 | Mouse monoclonal IgG1 | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:100 | Goat anti-mouse IgG488/647 |
| Gata4 | Rabbit polyclonal IgG | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:200 | Goat anti-rabbit IgG 568 |
| Irx4 | Mouse IgG+IgM | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | Undiluted | Goat anti-mouse IgG+IgM 488 |
| Cxcr4-647 | RatIgG2g, κ | 5% (wt/vol) BSA, 2% (vol/vol) goat serum | 1:50 | NA |
| Flk1-PE | RatIgG2a | 5% (wt/vol) BSA, 2% (vol/vol) goat serum | 1:20 | NA |
| cKit-cy5 | RatIgG2g, κ | 5% (wt/vol) BSA, 2% (vol/vol) goat serum | 1:50 | NA |
| α-Actinin | Mouse monoclonal IgM | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:250 | Goat anti-mouse IgM568 |
| Cardiac actin | Mouse monoclonal IgG1 | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:400 | Goat anti-mouse IgG488/647 |
| α/β-MHC | Mouse monoclonal IgG1, κ | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:200 | Goat anti-mouse IgG488/647 |
| MLC-2v | Rabbit polyclonal IgG | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:200 | Goat anti-rabbit IgG633 |
| MLC-2a | Mouse monoclonal IgG | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:200 | Goat anti-mouse IgG488/647 |
| Cx43 | Mouse monoclonal IgG1 | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:100 | Goat anti-mouse IgG488/647 |
| SM-MHC | Rabbit polyclonal IgG | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:250 | Goat anti-rabbit IgG568 |
| SMA | Mouse monoclonal IgG | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:400 | Goat anti-mouse IgG488/647 |
| CD31 | Rat monoclonal IgG2a | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:400 | Goat anti-rat IgG 568 |
| GFP | Rabbit polyclonal IgG or mouse monoclonal IgG | 5% (wt/vol) BSA, 2% (vol/vol) goat serum, 0.1% (vol/vol) Triton X-100 | 1:200 | Goat anti-rabbit IgG488/568 or Goat anti-mouse 488/568/647 |