Table 2.

Transposed DNA Amplification mix

Component	Volume (μL)
Transposed DNA (from Subheading 3.3 step 4)	10
Water	10
25 μM ATAC Primer 1	2.5
25 μM ATAC barcoded Primer 2*	2.5
2X NEBNext High Fidelity PCR Mix	25
Total	50

^*A different barcoded Primer 2 should be used for each library that is to be pooled into a single sequencing run.