Figure 3.

Rate of tethering of cells expressing wild-type or CD43TMD PSGL-1 on mP-selectin. CHO cells expressing wild-type or CD43TMD PSGL-1 were perfused over mP-selectin at 66 sites/μm² (A) or 31 sites/μm² (B). The number of cells that tethered to mP-selectin during the first 60 s was quantified and normalized by dividing by the number of cells delivered across the field of view in the focal plane of the substrate. The percentage of tethers that were transient or that were converted to rolling adhesion is also indicated. The data represent the mean ± SD of three experiments. At each wall shear stress, the difference in percentage of cells that converted to rolling adhesion between wild-type and CD43TMD PSGL-1 was significant at P < 0.05.