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. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: Oncogene. 2016 Sep 12;36(10):1374–1383. doi: 10.1038/onc.2016.301

Figure 1. MDM2C305Fmutation has no discernable effect on APC loss-induced small intestinal tumors.

Figure 1

A. Hematoxylin and eosin (H&E) staining of small intestine and colon isolated from eight month-old WT and Mdm2m/m mice. Scale bar = 200μm.

B. Ki-67 immunohistochemical (IHC) staining of proliferating cells in small intestine and colon isolated from eight month-old mice. Proliferating cells are located at the bottom of crypts in both the small intestine and colon. Scale bar = 200μm.

C. IHC staining of cleaved caspase-3 (CC-3) to probe for apoptotic cells (indicated by arrows) in small intestine and colon tissue isolated from eight month-old mice. Scale bar = 100μm.

D. mRNA expression of lgr5, lyz1, muc2 and chga in small intestine or colon from eight month-old WT or Mdm2m/m mice was analyzed by qRT-PCR. n=3 for each genotype. Error bars, ±SEM.

E. Small intestines were isolated from 15 week-old mice, and polyp numbers per mouse were counted under a dissection microscope (n=12 for each genotype).

F. The average cross sectional areas (CSA) of H&E stained small intestinal adenomas were determined quantitatively using ImageJ.

G. Kaplan-Meier survival curves for ApcMin/+; Mdm2+/+ (n=40) and ApcMin/+; Mdm2m/m (n=18) mice are shown. The median survival times did not differ significantly between the two genotypes.