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. Author manuscript; available in PMC: 2018 Feb 21.
Published in final edited form as: Cell Rep. 2017 Feb 21;18(8):1831–1839. doi: 10.1016/j.celrep.2017.02.002

Figure 3. Rab8 acts downstream of Stratum in polarized BM deposition.

Figure 3

(A–E) Lg-sections through egg chambers expressing Coll IV-GFP (green), stained for F-Actin (red) and DNA (blue). The FEs of egg chambers express strat RNAi (A), strat RNAi and Rab10CA (B), strat RNAi and Rab8CA (C–D) or strat RNAi and Rab8 (E) using tj-Gal4. (F) Quantification of BM mislocalization observed in A–D (n≥76; ****, p<0.0001; n.s., non-significant). (G) YFPMYC-Rab8 interacts with Strat-HA in ovary extracts. (H) Lg-section through egg chambers containing strat mutant FC clones (dashed line indicates clonal boundary; asterisks (*) specify homozygous mutant FCs) and stained for α1-Collagen IV (red) and DNA (blue). (I–J) Lg-sections through FE expressing Pcan-GFP (I, green) or Coll IV-GFP (J, green), stained for F-Actin (red) and DNA (blue) and expressing Rab8 RNAi (I) or Rab8DN (J). Bars, 10 μm.