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. Author manuscript; available in PMC: 2018 Sep 11.
Published in final edited form as: Curr Biol. 2017 Aug 17;27(17):2569–2578.e4. doi: 10.1016/j.cub.2017.07.013

Figure 2. Hierarchal relation among sam-4, arl-8 and unc-104.

Figure 2

(A-H) Image montages of the dorsal axons in sam-4(tm3828) (A-D) and arl-8(wy271) (E-H) expressing sam-4 cDNA (B and F), arl-8 cDNA (C and G) and unc-104 cDNA (D and H). sam-4, arl-8 and unc-104 cDNA were expressed using the mig-13 promoter (DA9-specific promoter). Dorsal synapses were visualized using Pitr-1∷gfp∷rab-3. 10 confocal images from each genotype were cropped, straightened, and aligned using Image J. Representative results from at least three independent lines are shown. Bar, 20 μm.

(I and J) Statistical analysis of suppressor mutants. (I) The number of puncta mis-accumulated to the commissure and (J) the length of the asynaptic region. Lines show median values, and each dot represents one animal. Kruskal–Wallis one-way ANOVA on ranks and Dunn's multiple comparisons test; compared to wild type, *P Value < 0.05; **Adjusted P Value < 0.01; ***Adjusted P Value < 0.001. n= 20 animals for each genotype. See also Figure S2.