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A, RBL cells were passively sensitized with sera of immunized mice, and mediator release was triggered by addition of antigen. B, Splenocytes of immunized animals cultured in the presence of antigen- and cytokine-producing cells were identified in ELISpot assays. C, Splenocyte supernatants were analyzed with a multiplex approach. P values of less than .05 were considered significant. *P < .05 and **P < .01.
